| North-South Bicycle Route |
| | Academy Street from the southern City boundary to Catharine Street |
| | Catharine Street from Academy Street to Cottage Street |
| | Cottage Street from Catharine Street to North Hamilton Street |
| | North Hamilton Street from Cottage Street to the former railroad bed |
| Cherry-Cannon Bicycle Route |
| | Kimball Road from the intersection with Yates Boulevard to Marian Avenue |
| | Marian Avenue from Kimball Road to Hooker Avenue |
| | Hooker Avenue from Marian Avenue to South Cherry Street |
| | South Cherry Street from Hooker Avenue to Cannon Street |
| | Cannon Street from South Cherry Street to Market Street |
| Fallkill Bicycle Route |
| | High Street from North Hamilton Street to Elm Place |
| | Elm Place from High Street to Zimmer Avenue |
| | Zimmer Avenue from Elm Place to Garden Street |
| | Garden Street from Zimmer Avenue to Brookside Avenue |
| | Brookside Avenue from Garden Street to Verrazano Boulevard |
| | Verrazano Boulevard from Brookside Avenue to Lower Mill Street |
| | Lower Mill Street from Verrazano Boulevard to Davies Place |
| Northside Bicycle Route |
| | Mansion Street from Corlies Avenue to Mansion Square Park |
| | Through Mansion Square Park along its east-west axis from North Clinton Street to North Hamilton Street |
| | Mansion Street from North Hamilton Street to North Bridge Street |
| | North Bridge Street Mansion Street to Mill Street |
| | Mill Street from North Bridge Street to Davies Place |
| | Davies Place from Mill Street to the Poughkeepsie Railroad Station |
| School Connector Bicycle Route |
| | Corlies Avenue from Mansion Street to King Street |
| | King Street from Corlies to Innis Avenue |
| | Innis Avenue King Street to Worrall Avenue |
| | Worrall Avenue from Innis Avenue to College Avenue |
| Smith Street Bicycle Route |
| | Smith Street from Mansion Street to the Morgan Lake parking area/Dutchess Rail Trail |
| Southside-Waterfront Bicycle Route |
| | Main Street from Waryas Park to Rinaldi Boulevard |
| | Rinaldi Boulevard from Main Street to Pine Street |
| | Pine Street Rinaldi Boulevard to Pine Street Spur |
| | Pine Street Spur from Pine Street to Columbia Street |
| | Columbia Street from Pine Street Spur to Young Street |
| | Young Street from Columbia Street to Reade Place |
| | Reade Place from Young Street to Lincoln Avenue |
| | Lincoln Avenue from Reade Place to Livingston Street |
| | Livingston Street from Lincoln Avenue to South Hamilton Street |
| | South Hamilton Street from Livingston Street to Platt Street |
| | Platt Street from South Hamilton Street to Loockerman Avenue |
| | Loockerman Avenue from Platt Street to Yates Boulevard |
| | Yates Boulevard from Loockerman Avenue to Kimball Road |
| | Kimball Road from Yates Boulevard to Bancroft Road |
| | Bancroft Road from Kimball Road to Ferris Lane |
| | Ferris Lane from Bancroft Road to Thornwood Drive |
| | Thornwood Drive from Ferris Lane to Monell Avenue |
| | Monell Avenue from Thornwood Drive to Wilbur Boulevard |
| Vassar College Bicycle Route |
| | College Avenue from the City boundary to West College Avenue |
| | West College Avenue from College Avenue to Circular Drive |
| | Circular Drive from West College Avenue to Hooker Avenue |
| City Transect Bicycle Route |
| | Hooker Avenue from the City boundary to Montgomery Street |
| | Montgomery Street from Hooker Avenue to Jefferson Street |
| | Jefferson Street from Montgomery Street to South Perry Street |
| | South Perry Street from Jefferson Street to Union Street |
| | Union Street from South Perry Street to South Clover Street |
| | South Clover Street from Union Street to North Clover Street |
| | North Clover Street from South Clover Street to Mill Street |
| | Mill Street from North Clover Street to Mount Carmel Place |
| | Mount Carmel Place from Mill Street to Delafield Street |
| | Delafield Street from Mount Carmel Place to the City boundary |